| 质粒名称: | HIP-flash (HOP-flash mutant) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 83466 |
| 存储实验室: | Barry Gumbiner |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3-basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4797 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 荧光素酶表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter,control TEAD reporter |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 213 bp |
| 基因突变: | -- |
| GenBank编号 | 7003, 8463,7005, 7004 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SacI(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | RVprimer3 |
| 3' 测序引物 | LucNrev |
|
文献引用方法 |
|
| 材料和方法部分: | HIP-flash (HOP-flash mutant) was a gift from Barry Gumbiner (Addgene plasmid # 83466 ; http://n2t.net/addgene:83466 ; RRID:Addgene_83466) |
| 参考文献部分: | Adhesion to fibronectin regulates Hippo signaling via the FAK-Src-PI3K pathway. Kim NG, Gumbiner BM. J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. Epub 2015 Jul 27. 10.1083/jcb.201501025 PubMed 26216901 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS HIP-flash (HOP-flash mutant) 5010 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Negative control for HOP-flash. 7xmut TEAD binding sites with
minimal promoter plus luciferase reporter gene.
ACCESSION .
VERSION .
KEYWORDS HIP-flash (HOP-flash mutant)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5010)
AUTHORS Kim NG, Gumbiner BM
TITLE Adhesion to fibronectin regulates Hippo signaling via the
FAK-Src-PI3K pathway.
JOURNAL J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025.
Epub 2015 Jul 27.
PUBMED 26216901
REFERENCE 2 (bases 1 to 5010)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5010
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 274..283
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 280..1932
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(346..364)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 1973..2094
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1984..2003)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2038..2057
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2342..2359)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2513..3101)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2593..2612)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3272..4132)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3895..3914
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4133..4237)
/gene="bla"
/label=AmpR promoter
rep_origin 4264..4719
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4351..4370)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4561..4582
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4850..4898
/note="synthetic polyadenylation signal"
misc_feature 4912..5003
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4952..4971
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg cccttggtgg
61 ggagagatgc gagagatgtc cctgtttgtg gtggggagag atgcgagaga tgtccctgtt
121 tgtggtgggg agagatgcga gagatgtccc tgtttgtggt ggggagagat gcgagagata
181 agggcgaatt ctgcagatta gagggtatat aatggatccg gtactcgaga tctgcgatct
241 aagtaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
1921 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
1981 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
2041 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
2101 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
2161 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag
2221 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
2281 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
2341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
2461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
2701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
2761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
2821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
2881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
2941 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
3001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
3061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
3121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
3181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
3241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
3301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
3421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
3541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
3601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
3661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
3721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
3781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
3841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
3901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
3961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
4021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
4081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
4141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
4201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
4261 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4321 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
4381 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4441 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
4501 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4561 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
4621 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
4681 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
4741 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
4801 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
4861 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
4921 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
4981 tgcaggtgcc agaacatttc tctatcgata
//
